Category: Propagation

MERICLONES: Clonal Aberrations

Author is Robert M. [Bert] Hamilton (Compiler), posted over 7 years ago From one piece of tissue, after about 25,000 plantlets have been proliferated the aberration rate becomes unacceptibly high, and almost invariably in the direction of poorer quality. AU79-197 Read More

MERICLONES: Numbers of Explants

Author is Robert M. [Bert] Hamilton (Compiler), posted over 7 years ago Commercial laboratories usually produce only 5000 explants before mericloning the mother plant again, but if the mother plant is known to be stable the production can rise to 20,000. Au81-165; Hort... Read More

DEFLASKING: Foolproof Method

Author is Robert M. [Bert] Hamilton (Compiler), posted over 7 years ago Soak seedling bark in very warm water for two hours, remove seedlings from flask and place in warm water to remove all agar from roots; grade them by size, pot each one gently and do not pack the b... Read More

DEFLASKING: For Beginners

Author is Robert M. [Bert] Hamilton (Compiler), posted over 7 years ago Acclimatize the flask for a few days in the immediate growing area preferably shaded and with an even temperature, not less than 60 dF (15 dC) for cool growing plants, avoid extremes, preferably ad... Read More

FLASKING FORMULAS: And Nutrient Solutions

Author is Robert M. [Bert] Hamilton (Compiler), posted over 7 years ago For an extensive list of formulas and their component chemicals or substances, refer to W1-589+ Read More

FLASKING FORMULAS: Curtis Medium

Author is Robert M. [Bert] Hamilton (Compiler), posted over 7 years ago For germination of Epipactis seeds; chemical components and application, refer to A82-168; OBII-243 Read More

FLASKING FORMULAS: For Orchid Seed Germination

Author is Robert M. [Bert] Hamilton (Compiler), posted over 7 years ago Instant solutions for dendrobium, cattleya, cymbidium, paphiopedilum, developed by scientists at Kyoto University using Hyponex, sugar, agar, water, apple juice, etc.; refer to A63-354+ Read More

FLASKING FORMULAS: Knudson-c Medium

Author is Robert M. [Bert] Hamilton (Compiler), posted over 7 years ago Monopotassium phosphate 0.25gm., calcium nitrate 1.00 gm., ammonium sulphate 0.50 gm., magnesium sulphate 0.25 gm., ferrous sulphate 0.025gm., manganese sulphate 0.0075 gm., agar 17.5 gm., sucrose ... Read More

FLASKING: Reflasking Alternative

Author is Robert M. [Bert] Hamilton (Compiler), posted over 7 years ago Use a 2-liter plastic pop bottle; heat the bottom stand in hot water to soften the adhesive and pull it off, cut off the top section of the pop bottle and invert it into the stand portion to form a... Read More

CLASSIFICATION: Family Tree

Author is Robert M. [Bert] Hamilton (Compiler), posted over 7 years ago For a one-page chart of all classified genera, refer to David Sander's book Orchids and their cultivation (7th ed., 1969) Read More