STERILIZATION: Of Pots and Media

Orchid Doctor

by Robert M. [Bert] Hamilton (Compiler)

Originally published in The Orchid Doctor in 1980 and 1988

Posted by Sys Admin over 3 years ago.

Article Blog   Article Index
Immersion in water heated to 162 dF (72 dC) for 10 minutes stopped the loss of seedlings from disease where fungicides had little if any effect. AU81-20
Share on Social Media:  
Log in to Add a Comment

More The Orchid Doctor Articles under the Heading Sterilization

Sterilization Comments
STERILIZATION: By Microwave Ovens Flasks with agar medium and stoppers can be qui ckly processed, but care is necessary, for details of two methods, refer to OR86-334 0
STERILIZATION: Compost With heat: heat the compost at 180 to 190 deg. F. for 30 minutes. A71-21 0
STERILIZATION: Cutting Tools Place in sodium hydroxide 20% solution for half a minute; in trisodium phosphate 15% solution for 15 to 30 minutes; in 5% clorox solution for five to 10 minutes. AU87(1)-16Use cheap cigaret te lighters to flame the blades before using. OIE86Sept-7 0
STERILIZATION: Loam or Leaf Mold With heat: dry the materials first, then heat at 250 deg.F. for 30 minutes to kill virus; or use a pressure cooker at 212 deg.F. for 10 to 15 minutes. A70-345 0
STERILIZATION: Pots With a pressure cooker: they should be sterilized this way as a matter of course, if of clay. A73-615 0
STERILIZATION: Tools With Clorox: use one part Clorox to four parts water. A73-615; use one part Clorox to nine parts water. A79-910; Clorox kills in five to ten minutes. A79-1258; A80-110With flame: a candle flame is slow and poor. A70-1099; an alcohol lamp is good but better if the blade is first dipped in alcohol. A70-1099; hold the blade in the flame of an alcohol lamp or a Bunsen burner or a blow torch and heat the metal short of white heat. A73-334; flame the blade in a propane burner until nearly red. A75-437With Physan 20: it is relatively slow and heat treatment is more reliable. A76-115; use it at 2 ttpg; it is not highly toxic to humans. A79-272; use at 2 ttpg. for five to 15 minutes. A79-272; OD76-122With sodium hydroxide (NaOH) use a 2% solution for two or three minutes. A70-427; caustic soda; use a 2% solution. A73-615; use a 2% solution. A79-910; a 2% solution kills virus in half a minute to two minutes; it is the fastest. A79-1258; A80-110With trisodium phosphate: use a 15% solution in w... 0
STERILIZATION: Tools (general) Heating knives to red hot can distemper the metal; use cheap knives with non-conducting handles; single razor blades are good but can cause nasty cuts and they deteriorate quickly from rusting, etc.; best yet is a long thin blade with sheet metal handle, a Japanese industrial knife "Nikken Sofutu Kamisori" available from supply firms; sterilize them by baking. OD68-148To kill Tobacco Mosaic Virus heat metal to boiling 212 deg.F. (100 deg.C) for at least one minute; to inactivate TMV in undiluted plant juice requires one minute heating at 96 deg.C. (204.8 deg.F.); for Cymbidium Orchid Mosaic Virus heat for 10 minutes at 65 deg.C. (117 deg.F.) C72-42 0
STERILIZATION: Tools -- with Chemicals A mix of formaldehyde and sodium hydroxide was once used but now just a 2% solution of the latter is used and is as good; it has a higher pH of 14 than the formaldehyde; the higher the pH the quicker the denaturization of virus. A69-902 0

New Topics

  1. Maria Delaney asked question Double spikes on oncidiums in category Oncidium Alliance
  2. Darrin Duling asked question unknown Oncidium intergeneric in category General Discussion
  3. Darrin Duling asked question need id on oncidium intergeneric in category General Discussion
  4. Kate McMillan started topic Horticultural Charcoal vs Activated Charcoal in category General Discussion
  5. Susan smith asked question Vanda Samsri Gory in category General Discussion

New Comments

  1. Melvin Waki commented on member plant Hwra. Lava Burst by Jeanne M Uzar
  2. Carol Holdren commented on member plant Den. Ruth Chun's Gemini by Melvin Waki
  3. Samuel Yanos commented on member plant Coel. rochussenii 'Waki' by Melvin Waki
  4. Mo Chen commented on member plant Den. longicornu by Mo Chen
  5. Yahoo Alex commented on member plant Habenaria Tanager by David Nickerson
  6. Adam Almeida commented on member plant Chz. Hsinying Pink Doll 'Hsinying' by Melvin Waki
  7. Alex Maximiano commented on member plant V. Boschii by Adam Almeida
  8. Carol Holdren commented on member plant Ar. graminifolia by Adam Almeida
  9. Melvin Waki commented on member plant Pol. neobenthamia by Adam Almeida
  10. Carol Holdren commented on member plant V. Nopporn White Diamond by Adam Almeida
  11. Melvin Waki commented on member plant V. Yip Sum Wah 'Flame' by Tanya Lam
  12. Tanya Lam commented on member plant Gra. stapeliiflora 'Waki' by Melvin Waki
  13. Gustavo Sánchez commented on orchid Lc. Blue Rose
  14. Carol Holdren commented on member plant Aerdv. Somsri Sunlight by Mary Lane
  15. Tanya Lam commented on member plant Cym. finlaysonianum by Tanya Lam
  16. Alex Maximiano commented on member plant Lc. Dinard 'Blue Heaven' by Melvin Waki
  17. Carol Holdren commented on member plant Rth. Yellow Bouquet 'Pam' by Melvin Waki
  18. Carol Holdren commented on member plant V. falcata var. Hisui by Tanya Lam
  19. David Nickerson commented on member plant Ctsm. Edgardo A. Pauneto 'Dark Stripes' by David Nickerson
  20. Carol Holdren commented on member plant Phal. violacea var. indigo 'Royal Blue' by Vickie Seibert