STERILIZATION: Compost

Orchid Doctor

by Robert M. [Bert] Hamilton (Compiler)

Originally published in The Orchid Doctor in 1980 and 1988

Posted by Sys Admin over 5 years ago.

Article Blog   Article Index
With heat: heat the compost at 180 to 190 deg. F. for 30 minutes. A71-21
Share on Social Media:  
Log in to Add a Comment

More The Orchid Doctor Articles under the Heading Sterilization

Sterilization Comments
STERILIZATION: By Microwave Ovens Flasks with agar medium and stoppers can be qui ckly processed, but care is necessary, for details of two methods, refer to OR86-334 0
STERILIZATION: Cutting Tools Place in sodium hydroxide 20% solution for half a minute; in trisodium phosphate 15% solution for 15 to 30 minutes; in 5% clorox solution for five to 10 minutes. AU87(1)-16Use cheap cigaret te lighters to flame the blades before using. OIE86Sept-7 0
STERILIZATION: Loam or Leaf Mold With heat: dry the materials first, then heat at 250 deg.F. for 30 minutes to kill virus; or use a pressure cooker at 212 deg.F. for 10 to 15 minutes. A70-345 0
STERILIZATION: Of Pots and Media Immersion in water heated to 162 dF (72 dC) for 10 minutes stopped the loss of seedlings from disease where fungicides had little if any effect. AU81-20 0
STERILIZATION: Pots With a pressure cooker: they should be sterilized this way as a matter of course, if of clay. A73-615 0
STERILIZATION: Tools With Clorox: use one part Clorox to four parts water. A73-615; use one part Clorox to nine parts water. A79-910; Clorox kills in five to ten minutes. A79-1258; A80-110With flame: a candle flame is slow and poor. A70-1099; an alcohol lamp is good but better if the blade is first dipped in alcohol. A70-1099; hold the blade in the flame of an alcohol lamp or a Bunsen burner or a blow torch and heat the metal short of white heat. A73-334; flame the blade in a propane burner until nearly red. A75-437With Physan 20: it is relatively slow and heat treatment is more reliable. A76-115; use it at 2 ttpg; it is not highly toxic to humans. A79-272; use at 2 ttpg. for five to 15 minutes. A79-272; OD76-122With sodium hydroxide (NaOH) use a 2% solution for two or three minutes. A70-427; caustic soda; use a 2% solution. A73-615; use a 2% solution. A79-910; a 2% solution kills virus in half a minute to two minutes; it is the fastest. A79-1258; A80-110With trisodium phosphate: use a 15% solution in w... 0
STERILIZATION: Tools (general) Heating knives to red hot can distemper the metal; use cheap knives with non-conducting handles; single razor blades are good but can cause nasty cuts and they deteriorate quickly from rusting, etc.; best yet is a long thin blade with sheet metal handle, a Japanese industrial knife "Nikken Sofutu Kamisori" available from supply firms; sterilize them by baking. OD68-148To kill Tobacco Mosaic Virus heat metal to boiling 212 deg.F. (100 deg.C) for at least one minute; to inactivate TMV in undiluted plant juice requires one minute heating at 96 deg.C. (204.8 deg.F.); for Cymbidium Orchid Mosaic Virus heat for 10 minutes at 65 deg.C. (117 deg.F.) C72-42 0
STERILIZATION: Tools -- with Chemicals A mix of formaldehyde and sodium hydroxide was once used but now just a 2% solution of the latter is used and is as good; it has a higher pH of 14 than the formaldehyde; the higher the pH the quicker the denaturization of virus. A69-902 0

New Topics

  1. Stefan Neher asked question Stale Data in category Site Navigation
  2. Penny Edwards asked question diseases in category Phalaenopsis
  3. Penny Edwards asked question diseases in category Phalaenopsis
  4. peter teller asked question please help with an id..... in category General Discussion
  5. Roberto Lizama started topic Help to ID this Epidendrum from Guatemala in category Species

New Comments

  1. Carol Holdren commented on topic "Stale Data" by Stefan Neher
  2. Robert H. Findlay commented on topic "seed vs. pollen parent " by Robert H. Findlay
  3. Carol Holdren commented on topic "Photo of MaryAnn Coyle or Laura Ann Coyle" by Dana Coyle
  4. Mary Lane commented on member plant Dendrobium QT Makani by Mary Lane
  5. Brandy France commented on member plant Chz. Hsinying Pink Doll 'Hsinyijng' by Adam Almeida
  6. Roberto Lizama commented on topic "Help to ID" by Roberto Lizama
  7. Carol Holdren commented on topic "Brsdm GILDED URCHIN ONTARIO ORCHID" by Diana King
  8. Carol Holdren commented on topic "Compact DEN SUPERBUM ORCHID MINEATURE " by Diana King
  9. Pam Lipscomb commented on member plant Blc. Oconee 'Mendenhall' by Jeanne M Uzar
  10. Carol Holdren commented on topic "Don't we meet in person in Kalamazoo, MI anymore?" by Conni Wireman
  11. Catherine Chance commented on topic "Searching for Erycina echinata " by Catherine Chance
  12. Carol Holdren commented on member plant Gom. Jiaho Queen 'Jiaho' by Robert Rand
  13. Pam Lipscomb commented on member plant Bsn. Maikai var. Louise by Sandi Block-Brezner
  14. Robert H. Findlay commented on member plant E. Rioclarense by Robert H. Findlay
  15. Susan LE commented on member plant QF Makani by Tanya Lam
  16. Pam Lipscomb commented on member plant Eny. Exotic Seashell by Steven Kirincich
  17. Pam Lipscomb commented on member plant Lc. Little Oliver by Steven Kirincich
  18. Susan LE commented on topic "Paph Harry Stage" by Key Stage
  19. Susan LE commented on scheduled event "Tamiami International Orchid Festival" by Miami Orchid Society
  20. Susan LE commented on article "Orchid Wiz - what will we do without you?" by Carol Holdren